Journal: Stem Cells International

Article Title: Vascularization of a Bone Organoid Using Dental Pulp Stem Cells

doi: 10.1155/2023/5367887

Figure Lengend Snippet: Comparison of the sprouting ability of BMSCs and DPSCs. (a) Photomicrographs of sprouting capillary structures formed by BMSCs and DPSCs with endothelial induction. (b) Quantitative analyses of the number of branches and (c) total branching length at corresponding time points. Scale bars: 200 μ m. ∗ p < 0.05; mean ± SD; n = 4.

Article Snippet: Human BMSCs derived from iliac crests (Lonza, Basel, Switzerland) and DPSCs isolated from adult third molars (Lonza) were cultured in growth medium (GM) comprising Dulbecco's modified Eagle's medium (DMEM; Wako, Osaka, Japan) supplemented with 20% fetal bovine serum (Thermo Fisher Scientific, MA, USA) and 1% penicillin-streptomycin (Sigma-Aldrich, MO, USA).

Techniques: Comparison

Journal: Stem Cells International

Article Title: Vascularization of a Bone Organoid Using Dental Pulp Stem Cells

doi: 10.1155/2023/5367887

Figure Lengend Snippet: Comparison of endothelial differentiation ability of BMSCs and DPSCs. mRNA expression of (a) VEGFA , (b) CXCL1 , and (c) Nanog after 1, 3, 7, and 14 days of endothelial induction. Different capital letters indicate significant differences among groups ( p < 0.05); mean ± SD; n = 4.

Article Snippet: Human BMSCs derived from iliac crests (Lonza, Basel, Switzerland) and DPSCs isolated from adult third molars (Lonza) were cultured in growth medium (GM) comprising Dulbecco's modified Eagle's medium (DMEM; Wako, Osaka, Japan) supplemented with 20% fetal bovine serum (Thermo Fisher Scientific, MA, USA) and 1% penicillin-streptomycin (Sigma-Aldrich, MO, USA).

Techniques: Comparison, Expressing

Journal: Stem Cells International

Article Title: Vascularization of a Bone Organoid Using Dental Pulp Stem Cells

doi: 10.1155/2023/5367887

Figure Lengend Snippet: Structure of the cell constructs after endothelial and osteogenic induction. (a) HE staining, (b) von Kossa staining, and (c) fluorescence imaging of cell constructs with varying ratios of BMSCs and DPSCs. White arrows indicate a capillary-like hollow structure in the mineralized 80B/20D constructs. Dotted lines indicate the outline of the cell constructs. Scale bars: 50 μ m.

Article Snippet: Human BMSCs derived from iliac crests (Lonza, Basel, Switzerland) and DPSCs isolated from adult third molars (Lonza) were cultured in growth medium (GM) comprising Dulbecco's modified Eagle's medium (DMEM; Wako, Osaka, Japan) supplemented with 20% fetal bovine serum (Thermo Fisher Scientific, MA, USA) and 1% penicillin-streptomycin (Sigma-Aldrich, MO, USA).

Techniques: Construct, Staining, Fluorescence, Imaging

Journal: Stem Cell Research & Therapy

Article Title: Rankl genetic deficiency and functional blockade undermine skeletal stem and progenitor cell differentiation

doi: 10.1186/s13287-024-03803-3

Figure Lengend Snippet: Impact of Denosumab treatment on the mineralization capacity of human BMSCs. a Representative image of ARS-stained cultures of human BMSCs from healthy donors after 2 weeks of culture either in basal medium (CTR) or in OIM alone or in OIM + isotype control (OIM + ISO) or in OIM + Denosumab (OIM + Dmab); in all the conditions, the culture medium was changed twice a week. At the end, the mineral deposition was quantified and indicated by Abs reading at 405 nm. Scale bar: 500 μm. Higher magnifications are provided in Figure . b Gene expression analysis of representative osteogenic genes in human BMSCs in the different treatment conditions indicated; normalization on 18 S. Results are expressed as Arbitrary Units (A.U.). All the data are represented as mean ± SEM. * p < 0.05; a: Friedman test; b: Kruskal-Wallis test. CTR: control. OIM: Osteogenic Induction Medium. ISO: isotype control. Dmab: Denosumab

Article Snippet: Human BMSCs from young healthy individuals of both sexes were purchased from Stem Cell Technologies (cat. #70,071).

Techniques: Staining, Control, Expressing